Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction

Charles Thill; P. Bryon Backenson; Melissa A. Prusinski; Sarah J. Kogut; Joon-Hak Lee; James L. Coleman

doi:10.1603/0022-2585(2005)042[0694:DOBMDI]2.0.CO;2

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Charles Thill,¹ P. Bryon Backenson,² Melissa A. Prusinski,³ Sarah J. Kogut,³ Joon-Hak Lee,^3,4 James L. Coleman¹

¹State of New York Department of Health, Centers for Molecular Medicine, Stony Brook University, Stony Brook, NY 11794-5120
²State of New York Department of Health, Arthropod-Borne Disease Program, Corning Tower, Room 632, Empire State Plaza, Albany, NY 12237
³State of New York Department of Health, Arthropod-Borne Disease Program, Hudson Valley Community College, Troy, NY 12180
⁴Current address: The Department of Environmental and Occupational Health, School of Public Health, University of North Texas-Health Science Center, Fort Worth, TX 76107

Abstract

A rapid and sensitive protocol using Chelex 100 resin for DNA extraction and the polymerase chain reaction for detection of Babesia microti Franca within its vector Ixodes scapularis Say is described. The level of detection of this protocol was determined to be the approximate equivalent of one parasite.

Citation Download Citation

Charles Thill, P. Bryon Backenson, Melissa A. Prusinski, Sarah J. Kogut, Joon-Hak Lee, and James L. Coleman "Detection of Babesia microti DNA in Ixodes scapularis (Acari: Ixodidae) by Use of Chelex 100 Resin and Polymerase Chain Reaction," Journal of Medical Entomology 42(4), 694-696, (1 July 2005). https://doi.org/10.1603/0022-2585(2005)042[0694:DOBMDI]2.0.CO;2

Received: 4 October 2004; Accepted: 2 March 2005; Published: 1 July 2005